匡汉晖

文章来源：华中农业大学时间：2014-06-24 15:46:53

【个人简介】

男，1967年生于江西泰和。现为华中农业大学园艺林学学院蔬菜系教授，

博士生导师。2008年受聘为长江学者特聘教授，

【个人履历】

园艺植物生物学教育部重点实验室常务副主任。

1984-1988年江西农业大学学习，获学士学位

1988-1991年北京林业大学学习，获硕士学位

1994-1998年在美国佐治亚大学(University of Georgia)学习，获博士学位

1998-2007年分别在以色列海法大学(University of Haifa)、美国加州大学戴维斯分校(University of California, Davis)和伯克莱分校(University of California, Berkeley)从事蔬菜抗病研究工作

2008年受聘为长江学者特聘教授，现为华中农业大学园艺林学学院蔬菜系教授，博士生导师

【主要研究领域及成果】

研究领域

植物抗病与分子进化

研究内容

茄科植物的抗病基因。

内容包括抗病基因（NBS-LRR）的生物信息学、遗传定位及克隆、分子进化、比较基因组学、功能基因组学。　1．基因组学：利用生物信息学方法分析烟草、西红柿和土豆基因组中的所有NBS-LRR基因。对这些抗病基因进行遗传定位、比较基因组学和分子进化的研究。利用实验手段了解抗病基因进化的规律。

2．抗病基因快速克隆方法的建立：通过对抗病基因的序列分析，找出抗病基因的保守序列，设计几个人工微RNA用以沉默大部分NBS-LRR基因，同时设计一套VIGS沉默载体沉默基因组中的绝大多数抗病基因。这套分析系统将极大提高植物抗性的遗传鉴定，加快抗病基因的克隆，尤其是QTL抗病基因和抗虫基因的克隆。这套系统将首先在烟草中进行，试验成功后再推广到其它蔬菜和农作物上。我们同时利用第二代高通量测序技术来快速定位和克隆基因。以上技术成熟后将应用到番茄青枯病和黄化曲叶病的抗性资源筛选和抗性基因克隆中。

3．烟草与TMV的相互作用。烟草属对TMV抗性的进化；不同野生烟草对TMV抗性的分子机理；用实验的方法了解抗病基因N的突变规律；TMV接种后无症状性状的遗传和分子机制。

【代表性论文】

1．Luo， S., Peng, J., Li, K., Wang, M., Kuang, H.（2011）Contrasting evolutionary patterns of the Rp1 resistance gene family in different species of Poaceae. Mol. Bio. Evol.　 28: 313-325

2．Kuang, H. Padmanabhan, C., Li, F. , Kamei, A., Bhaskar, P. B., Ouyang, S., Jiang, J., Buell, C. R., and Baker B. (2009) Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: New functional implications for MITEs. Genome Research, 19: 42-56.

3．Kuang, H., J. van Eck，H., Sicard，D., Michelmore, R. and Nevo, E. (2008) Evolution and genetic population structure of prickly lettuce (Lactuca serriola) and its RGC2 resistance gene cluster. Genetics, 178: 1547-1558.

4．Kuang, H., Caldwell, K., Meyers, B. and Michelmore, R. (2008) Frequent sequence exchanges between Homologs of RPP8 in Arabidopsis are not necessarily associated with genomic proximity. Plant Journal, 54: 69-80.

5．Zhang, L., Mojtahedi, H., Kuang, H., Baker, B. and Brown, C.R. (2007) The use of STS markers in the marker-assisted selection of Columbia root-knot nematode resistance introgressed from Solanum bulbocastanum. Crop Science, 47: 2021-2026

6．Kuang, H., Ochoa, O., Nevo E., and Michelmore R. (2006) The resistance gene Dm3 is infrequent in natural populations of Lactuca serriola due to deletions and frequent gene conversions. Plant Journal, 47: 38-48

7．Kuang, H., F. Wei, M. Marano, U. Wirtz, X. Wang, J. Liu, W. Shum , J. Zaborsky, L. J. Tallon, W. Rensink, S. Lobst, P. Zhang, C. Tornqvist, A. Tek, J. Bamberg, J. Helgeson, W. Fry, F. You, M. Luo, J. Jiang, R. C. Buell and B. Baker. (2005) The R1 resistance cluster contains three groups of independently evolving, Type I R1 homologues and shows substantial structural variation among haplotypes of Solanum demissum. Plant Journal, 44: 37-51

8．Huang, S., van der Vossen, E.A.G, Kuang, H., Vleeshouwers, V.G.A.A., Zhang, N., Borm, T.J.A., van Eck, H.J., Baker, B., Jacobsen, E. and Visser, R.G.F. (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant Journal, 42: 251-261

9．Kuang, H., Woo, S., Meyers, B., Nevo, E., Michelmore, R. (2004) Multiple genetic processes result in heterogeneous rates of evolution within the major cluster disease resistance genes in lettuce. Plant Cell, 16: 2870-2894.

10．Meyers, B., Kozik, A., Griego, A., Kuang, H., R.W. Michelmore (2003). Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell 15: 809-834.

11．Song, J., Bradeen, J.M., Naess, S.K., Raasch, J.A.,Haberlach, G.T., Wielgus S.M.,Liu, J., Kuang, H., Austin-Phillips, S., Buell, C.B., Helgeson, J.P., and Jiang, J. (2003) Gene RB from Solanum bulbocastanum confers broad spectrum resistance against potato late blight pathogen Phytophthora infestans. PNAS 100: 9128-9133.

12．Kuang, H., Richardson, T., Carson, P. and Bongarten, B. (1999) Genetic analysis of inbreeding depression in Pinus radiata D. Don. II. Genetics of viability genes. Theor. Appl. Genet. 99: 140-146.

13．Kuang, H., Richardson, T., Carson, S., Wilcox, P. and Bongarten, B. (1999) Genetic analysis of inbreeding depression in Pinus radiata D. Don. I. Genetic map with distorted markers. Theor. Appl. Genet. 98: 697-703.

14．Kuang, H., Rrchardson, T., Carson, S., and Bongarten, B. (1998) An allele responsible for seeding death in Pinus radiata D. Don. Theor. Appl. Genet. 96: 640-644.